User:Robert J. Schiemann/Notebook/Lipsick Lab/2009/04/22

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Spermatogenesis

  • Turn [FM7Ag/Y; FRT42D/CyoAg] stocks for experimental and control crosses.
  • Dissect ~30 gfp-h2au into 3 separate vials.
    • Potentially 1:10, 1:50, 1:100. Dissect ~30 flies. 10 per concentration.
    • Fix 30' in 4% Formaldehyde, PBS-T at RT.
    • Wash 4x5' in PBS-T at RT
    • Block in 2% NGS O/N