User:Robert J. Schiemann/Notebook/Lipsick Lab/2009/04/22

Drosophila melanogaster Genetics	Main project page Previous entry      Next entry
Spermatogenesis Turn [FM7Ag/Y; FRT42D/CyoAg] stocks for experimental and control crosses. Dissect ~30 gfp-h2au into 3 separate vials. Potentially 1:10, 1:50, 1:100. Dissect ~30 flies. 10 per concentration. Fix 30' in 4% Formaldehyde, PBS-T at RT. Wash 4x5' in PBS-T at RT Block in 2% NGS O/N