User:Robert J. Schiemann/Notebook/Lipsick Lab/2009/04/17

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Spermatogenesis

  • [GFP-myb, RFP-H2Au]
    • Wash 3x15' in PBS-T
    • Incubate w/ 1:500 dilution of a-Mouse IgG 633nm (Goat antibody) for 2 hours in NGS.
    • Wash 3x15' in PBS-T
    • Mount
  • Collect virgins and set up cross