User:Patrick Hampson/Notebook/chem471/2016/11/15

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UV-VIS

Sample Preparation

BSA UV-VIS 0.0625 11-15.PNG

BSA UV-VIS 0.5 11-15.PNG

BSA UV-VIS 1.25 11-15.PNG

BSA UV-VIS AUNP pH11 11-15.PNG

Data

BSA UV-VIS Ag -Fruc-0.0625 absorbance.PNG

The graph above contains our absorbance for Silver [Fructose]=0.0625 mM solution. Only pH 11 formed significant nanoparticles. pH7 and 8 also had some absorbance around the range we expect nanoparticles as well. The shoulder at 270 nm was apparent at with the pH 11 solution, and could be made out for the pH 7 and 8 solutions as well.

BSA UV-VIS Ag -Fruc-0.0625 530absorbance.PNG

The graph above shows the absorbance at wavelength 530 nm. It shows that pH 11 was the only solution to make significant nanoparticles (which is still rather low compared to previous trials). The absorbance for pH 7 and 8 was minor.

BSA UV-VIS -Fruc-0.5 absorbance.PNG

The graph above shows our UV-VIS spectra for our AGNP, NO BSA, fructose concentration of 0.5 This was a very interesting spectra. pH 10 has the highest absorbtion. pH 11 and 12 are very low in comparison to the other samples, while pH 4 is essentially a baseline throughout. pH 5,6,8, and 9 are clustered together. pH 7 is slightly lower than that cluster. All trials show the shoulder at 270 nm, with the exception of pH4 which is too low to show anything.

BSA UV-VIS -Fruc-0.5 530absorbance.PNG

The graph above shows the absorbance at 530 nm for our 0.5 fructose, no BSA silver solution. It reiterates the trends seen in the previous figure. no surprises.

BSA UV-VIS NoBAS Ag -Fruc-0.125 absorbance.PNG

The above graph shows the absorbance of our 0.125 Fructose, no BSA silver solution. pH 11 and 12 had the highest absorption. pH 10 was also significantly higher than the other trials which were clustered. pH 5,6,7,8, and 9 were the cluster, with pH 6 and 9 being somewhat higher and separated. pH 4 was once again essentially a baseline. There was a strong 270 nm shoulder for all trials.

BSA UV-VIS Ag -Fruc-1.25 530absorbance.PNG

The graph above shows the absorbance at 530 nm. It summarizes the trends seen in the previous figure. pH 11 and 12 were the highest, followed by pH 10. pH 6, 9 and also 8 had some nanoparticles present.

BSA UV-VIS NoBSA -Fruc-vary absorbance.PNG

This graph shows our absorbance of pH 11 AUNP solutions, all with varying fructose concentrations. All samples are right around the same absorbance, with the exception of the 0.75 nM fructose sample, which is significantly lower. In addition to a 270 shoulder, there also appears to be a minor 370 nm shoulder present in all samples.

BSA UV-VIS NoBSA -Fruc-vary 530absorbance.PNG

The graph above shows the absorbance of our varying fructose samples at 530 nm. It reiterates that 0.75 has the lowest absorbance.