User:Patrick Hampson/Notebook/chem471/2016/11/08

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Ocean Optics pH10

Sample Prep

BSA Ocean Optics

  1. [Au]=0.25 mM
  2. [BSA]=3.125 uM
  3. Vf=3000 uL

Stock Concentrations 161108

  1. [BSA]= 55.1 uM
  2. [Au]= 3.45 mM
  3. [NaOH]= 1 mM

Volumes for making the solution:

  1. 170.15 uL BSA
  2. 217.39 uL Au
  3. 2312.46 uL H2O
  4. 300 uL NaOH


The graph above displays the absorbance over time for our pH 10 solution. As previously seen, the beginning (0-4000 seconds) is exponential. The middle (4000-8000 seconds) is linear. The end (8000-~11000 seconds) is logarithmic.

The graph above displays the absorbance at wavelength 535.15nm versus time. As we have previously seen in every other parallel comparison, the graph mimics the general absorbance graph.

The graph above displays the maximum wavelength over time. There is a consistent maximum of 450/500 until about 3800 seconds (the point we observed a spike in pH8 and pH 9 previously). The maximum then spikes to the neightborhood of 540 ( slightly lower than we have seen in pH8 and 9). It remains there for the remainder of the experiment.