User:Patrick Hampson/Notebook/chem471/2016/09/21

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UV-Vis 2016921 Solution Preparation Stock Solutions: 14.8 mM Fructose 49.1 mM BSA 3.73 mM Au 1mM HCL 1mM NaOH 1M NaOH 0.75 mM Fructose pH μL of 1 mM HClstock μL of 1 mM NaOHstock μL of 1 M NaOHstock μL of AuCl3stock μL of Fructosestock μL of BSAstock μL of Water 4 500 0 0 335 253 316 3596 5 50 0 0 335 253 316 4046 6 5 0 0 335 253 316 4091 7 0 0 0 335 253 316 4096 8 0 5 0 335 253 316 4091 9 0 50 0 335 253 316 4046 10 0 500 0 335 253 316 3596 11 0 0 5 335 253 316 4091 12 0 0 50 335 253 316 4046 1.25 mM Fructose pH μL of 1 mM HClstock μL of 1 mM NaOHstock μL of 1 M NaOHstock μL of AuCl3stock μL of Fructosestock μL of BSAstock μL of Water 4 500 0 0 335 422 316 3427 5 50 0 0 335 422 316 3877 6 5 0 0 335 422 316 3922 7 0 0 0 335 422 316 3927 8 0 5 0 335 422 316 3922 9 0 50 0 335 422 316 3877 10 0 500 0 335 422 316 3427 11 0 0 5 335 422 316 3922 12 0 0 50 335 422 316 3877 After samples were prepared, they were placed in the thermooven at 80 degrees Celsius for four hours. Data The graph above displays the absorbance of 0.75 mM Fructose samples of pH 4-12 across the 200 nm to 800 nm range. The chart above shows the absorbance of 0.75 mM Fructose samples of pH4-12 at 530 nm. The graph above displays the absorbance of 1.25 mM Fructose samples of pH 4-12 across the 200 nm to 800 nm range. The chart above shows the absorbance of 1.25 mM Fructose samples of pH4-12 at 530 nm. Observations As previously seen, the samples of pH 11 and 12 were clear. As expected, they had significantly lower absorbance.