User:Patrick Hampson/Notebook/chem471/2016/09/20

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Fluorescence 2016920 Sample Preparation BSA Fluorescence Solution pH 5 [Au]=0.25 mM [BSA]=3.125 uM Vf=3000 uL Stock Concentrations 160920 [BSA]= 49.1 uM [Au]= 3.73 mM [HCl]= 1mM Volumes for making the solution: 30 uL HCl 190.936 uL BSA 147.63 uL Au 2631.42 uL H20 Results This graph shows the Fluorescence intensity expelled from the pH 5 sample (0.25 mM Au, 3.125 BSA) over time (as it was heated by the machine). It is apparent that the fluorescence intensity increases in time, as the sample was heated. This graph illustrated the emission maximums over time. The emissions increased until about 20 minutes into the experiment. After that the maximum emission was fluctuating just above 420 nm. The graph above shows the integrated intensity of the sample over time. The curve appears to take a logarithmic patter. The Fluorescence increases quickly in the initial 20 minutes, reaching ~7400 nm. After that the Fluorescence continues to increase, but at a significantly slower rate, barely exceeding 9000 nm by the end of the experiment. UV-Vis Sample Preparation for tomorrow Stock Solutions: 14.8 mM Fructose 49.1 mM BSA 3.73 mM Au 1mM HCL 1mM NaOH 1M NaOH 0.75 mM Fructose pH μL of 1 mM HClstock μL of 1 mM NaOHstock μL of 1 M NaOHstock μL of AuCl3stock μL of Fructosestock μL of BSAstock μL of Water 4 500 0 0 335 253 316 3596 5 50 0 0 335 253 316 4046 6 5 0 0 335 253 316 4091 7 0 0 0 335 253 316 4096 8 0 5 0 335 253 316 4091 9 0 50 0 335 253 316 4046 10 0 500 0 335 253 316 3596 11 0 0 5 335 253 316 4091 12 0 0 50 335 253 316 4046 1.25 mM Fructose pH μL of 1 mM HClstock μL of 1 mM NaOHstock μL of 1 M NaOHstock μL of AuCl3stock μL of Fructosestock μL of BSAstock μL of Water 4 500 0 0 335 422 316 3427 5 50 0 0 335 422 316 3877 6 5 0 0 335 422 316 3922 7 0 0 0 335 422 316 3927 8 0 5 0 335 422 316 3922 9 0 50 0 335 422 316 3877 10 0 500 0 335 422 316 3427 11 0 0 5 335 422 316 3922 12 0 0 50 335 422 316 3877 After the samples were prepared, they were left to cook in the thermooven at 80 degrees celcius for four hours.