User:Patrick Hampson/Notebook/chem471/2016/09/14

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Ocean Optics 2016918

Sample Preparation

  1. Stocks
    1. 4.57 mM Au
    2. 53.3 mM BSA
    3. 0.001 mM HCl
    4. DI Water
  1. Volumes Added to Generate Sample
    1. 175.89 uL BSA
    2. 164.11 uL Au
    3. 30 uL HCl
    4. 2629.99 uL H20

Protocol

Detailed protocol for Ocean Optics can be found in my journal from 9/7/2016 The sample was prepared and run through the Ocean Optics instrument. Data of the emissions was compiled once every two minutes for three hours.

Data

Ocean Optics Ph 5 part 1 160928.PNG

The graph above illustrates the maximum absorbance over time. There is a clear increase in the maximum absorbance as time goes on.

Ocean optics ph 5 part 2 160928.PNG

The graph above displays the maximum wavelength over time. There is a consistent maximum wavelength of approximately 445 nm until about 2800 seconds. Then there is a sharp peak followed by a seemingly inverse valley. Then the maximum wavelength sharply increases to about 335 nm, where it plateaus and is held relatively consistent for the remainder of the experiment.

Ocean optics ph 5 part 3 160928.PNG

This graph depicts the absorbance at 535.15 nm over time. It looks nearly identical to the maximum absorbance over time plotted above, which is expected.