User:Patrick Hampson/Notebook/chem471/2016/09/13

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BSA UV-Vis The samples made in the previous lab period were flawed. Dr. Hartings remade the samples following these steps: (Link says unwritten). Stock Solutions Used AuCl3 mass = 34.62 mg 25.0 mL volumetric flask MW = 303.3 g/mol concentration = 4.57 mM Bovine Serum Albumin mass = 35.62 mg 10.0 mL volumetric flask MW = 66776 g/mol concentration = 53.3 mM Lysozyme 33.32 mg of lysozyme 10.0 mL water in volumetric flask MW = 14307 g/mol concentration = 232.9 μM Myoglobin 32.23 mg of myoglobin water to 10.0 mL in volumetric flask MW = 16951 g/mol concentration = 137 μM Protocol Detailed versions of the protocol for UV-Vis can be found in earlier notebooks. Samples were prepared with an identical concentration of gold. The pH of the solutions were fixed to be 4,5,6,7,8,9,10,11,12 using 1 mM HCL. The solutions were placed in a cuvette and run through the fluorimeter. Data This graph shows the absorbance of Fructose conc. 0.125 mM, pH 4-12 across 200-800 nm. This graph shows the absorbance of Fructose conc. 0.25 mM, pH 4-12 across 200-800 nm. This graph shows the absorbance at peak wavelength 530nm for Fructose concentration 0.125 mM (pH 4-12). This graph shows the absorbance at peak wavelength 530nm for Fructose concentration 0.25 mM (pH 4-12). Analysis Similar to the UV-Vis trial the group previously conducted, the samples of pH 11 and 12 (for both concentrations of fructose) had significantly lower absorbance than that of the samples with pHs 4-10. Fructose 0.25 with pH 11 and 12 had a very faint pink coloration to them. Samples with pH 4-10 all had similar absorbance across the spectrum. Max absorbance at 530 nm was also similar. Notes 0.125 mM Fructose, 0.25 mM Au samples of pH 4-12. 0.25 mM Fructose, 0.25 mM Au samples of pH 4-12.