Objectives
The objective for today is to analyze protein-AuNP solutions prepared on 11/06/2013 using atomic absorption spectroscopy
AA Spectroscopy
- The general protocol for atomic absorption described on 11/05/2013 was followed. The samples were centrifuged for 30minutes prior to analysis.
Table 1. Standard Au Solution preparation from 1000μg/mL AA Au gold standard solution
| Concentration(μg/mL)
|
Volume of stock Au solution(mL)
|
Volume Deionized water added(mL)
|
| 10 |
0.1 |
9.9
|
| 20 |
0.2 |
9.8
|
| 30 |
0.3 |
9.7
|
| 40 |
0.4 |
9.6
|
| 50 |
0.5 |
9.5
|
Table 2. AA spectroscopy AuNP-protein solutions observations
| Au:Lysozyme Ratio
|
Appearance of solution
|
Au:Hemoglobin Ratio
|
Appearance of solution
|
| 5 |
Colorless |
30 |
Lightly colored
|
| 10 |
Lightly colored |
50 |
Lightly colored
|
| 15 |
Colored darkly |
70 |
Darkly colored
|
| 20 |
Colored |
90 |
Darkly colored
|
| 25 |
Colored |
110 |
Colorless
|
| 30 |
Colored |
130 |
Colorless
|
| 35 |
Colored |
150 |
Colorless
|
| 40 |
Colorless |
170 |
Colorless
|
| 45 |
Colorless |
190 |
Colorless
|
| 50 |
Colorless |
210 |
Colorless
|
- All of the solutions, if stated as colored, had a purplish color.
Figure 1. Absorbance spectra of AuNP Lysozyme solutions
Figure 2. Absorbance Spectra of AuNP-Hemoglobin solutions
Figure 3. Calibration Curve for Gold Standards
Figure 4. Concentration vs. Absorbance Au-Hemoglobin solutions
Figure 5. Concentration vs. Absrobance AuNP-Lysozyme solutions
Notes
- The AuNP-protein solutions prepared on 10/30/2013 will be analyzed during the next period. These samples contained a smaller volume (3.5mL) than the other volumes being centrifuged and as such will be analyzed during the next lab period after being centrifuged
| 
Project name
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