User:Matt Hartings/Notebook/Photosynthesis/2014/09/26

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Samples and Prep

sample 1

  1. Stock solutions:
    1. Myoglobin
      1. No myoglobin for this run
    2. OPD
      1. 2.5mg OPD in 2.5mL methanol
    3. H2O2
      1. 0.114mL 30% H2O2 in 0.887mL methanol
  2. Experimental Run
    1. Sample chamber set to 25C and to stir
    2. N2 run through the cuvette holder to reduce condensation on cuvette
    3. 2.025mL OPD stock solution
    4. 0.75mL H2O2 stock solution
      1. Start Data Collection
      2. 1ms Integration
      3. 10 scan average
      4. Wait 1 minute after each scan
      5. Stop after 2 hours
    5. Files saved as: OPD_H2O2_meoh_25_SCANNUMBER.txt
    6. 0.225mL methanol
    7. This is denoted in the data file for the third scan