User:Matt Hartings/Notebook/Photosynthesis/2014/10/14

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Samples

sample 1

  1. Stock solutions:
    1. Myoglobin
      1. 15.7mg Mb lyophilized from KCl/phosphate in 1mL ethanol
    2. OPD
      1. 2.6mg OPD in 2.6mL ethanol
    3. H2O2
      1. 0.114mL 30% H2O2 in 0.887mL ethanol
  2. Experimental Run
    1. Sample chamber set to 25C and to stir
    2. N2 run through the cuvette holder to reduce condensation on cuvette
    3. 2.025mL OPD stock solution
    4. 0.75mL H2O2 stock solution
      1. Start Data Collection
      2. 1ms Integration
      3. 10 scan average
      4. Wait 1 minute after each scan
      5. Stop after 2 hours
    5. Files saved as: Mb_OPD_H2O2_etoh_phosphate_25_SCANNUMBER.txt
    6. 0.225mL Mb/ethanol mix
    7. This is denoted in the data file for the third scan

sample 2

  1. Stock solutions:
    1. KCl Phosphate
      1. 15.2mg lyophilized KCl/phosphate in 1mL methanol
    2. OPD
      1. 2.8mg OPD in 2.8mL methanol
    3. H2O2
      1. 0.114mL 30% H2O2 in 0.887mL methanol
  2. Experimental Run
    1. Sample chamber set to 25C and to stir
    2. N2 run through the cuvette holder to reduce condensation on cuvette
    3. 2.025mL OPD stock solution
    4. 0.75mL H2O2 stock solution
      1. Start Data Collection
      2. 1ms Integration
      3. 10 scan average
      4. Wait 1 minute after each scan
      5. Stop after 2 hours
    5. Files saved as: KCl_OPD_H2O2_meoh_25_SCANNUMBER.txt
    6. 0.225mL KCl/phosphate in methanol
    7. This is denoted in the data file for the third scan