User:Matt Hartings/Notebook/Photosynthesis/2014/09/25

Protein Re-engineering

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Objective Learn how to maintain an OpenWetWare Notebook. Samples sample 1 Stock solutions: Myoglobin 15.2mg Mb lyophilized from KCl/phosphate in 1mL methanol OPD 2.5mg OPD in 2.5mL methanol H2O2 0.114mL 30% H2O2 in 0.887mL methanol Experimental Run Sample chamber set to 25C and to stir N2 run through the cuvette holder to reduce condensation on cuvette 2.025mL OPD stock solution 0.75mL H2O2 stock solution Start Data Collection 1ms Integration 10 scan average Wait 1 minute after each scan Stop after 2 hours Files saved as: OPD_H2O2_meoh_25_SCANNUMBER.txt 0.225mL Mb/Methanol mix This is denoted in the data file for the third scan sample 2 Stock solutions: KCl Phosphate 15.2mg lyophilized KCl/phosphate in 1mL methanol OPD 2.8mg OPD in 2.8mL methanol H2O2 0.114mL 30% H2O2 in 0.887mL methanol Experimental Run Sample chamber set to 25C and to stir N2 run through the cuvette holder to reduce condensation on cuvette 2.025mL OPD stock solution 0.75mL H2O2 stock solution Start Data Collection 1ms Integration 10 scan average Wait 1 minute after each scan Stop after 2 hours Files saved as: KCl_OPD_H2O2_meoh_25_SCANNUMBER.txt 0.225mL KCl/phosphate in methanol This is denoted in the data file for the third scan