Objective
Acquire fluorescence scans of lysozyme (pH 6) and myoglobin (pH 5) at 80C.
Lysozyme Fluorescence
3 mL Sample
- 0.5 μM BSA
- Stock Concentration 210 μM
- Volume Added: 7.1 μL
- pH 6
- Stock Concentration 1 mM HCl
- Volume Added: 3 μL
- Volume water added: 2990 μL
UV-Vis of sample
Evidence here of photobleaching of the fluorophore
Fluorescence scans
- Acquisition information
- Excitation: 290 nm, 10 nm slit width
- Emission: 310-540 nm, 10 nm slit width
- 100 nm/minute
- 61 measurements, 180 second delay between measurements
Myoglobin Fluorescence
3 mL Sample
- 12.5 μM BSA
- Stock Concentration 160 μM
- Volume Added: 234 μL
- pH 5
- Stock Concentration 1 mM HCl
- Volume Added: 30 μL
- Volume water added: 2736 μL
UV-Vis of sample
Evidence of protein aggregation (cloudy solution)
Fluorescence scans
- Acquisition information
- Excitation: 290 nm, 10 nm slit width
- Emission: 310-540 nm, 10 nm slit width
- 100 nm/minute
- 61 measurements, 180 second delay between measurements
Data
- Add data and results here...
Notes
This area is for any observations or conclusions that you would like to note.
Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.
|