User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2014/03/10

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Objective

I was looking at the UVVis data for HRP AuNPs today. I noticed that the student run data didn't qualitatively match with what the solutions looked like. The students all centrifuged their samples (30 min max speed) before taking their data (both AA and UV Vis). I wanted to take AA and UV Vis data for uncentrifuged samples. I also took data of centrifuged samples (with the remaining sample after AA).

Description

  1. Add experimental record here. Include what, how, and why...

Data

UV-Vis Data - uncentrifuged

Taking the 70, 150, and 190 samples that I made here, I ran UV Vis. I noted that the tube for the 150 sample was tinged a slight purple color, indicating that some AuNPs had adsorbed to its surface. I expect a slight diminishing of the signal for both UVVis and AA for this sample.

20140310 HRPAuNPuc UVVis.png

AA - uncentrifuged

Using the same samples as above ... I also made 6 standard concentrations for a calibration curve

  1. 0
    1. 10mL of water
  2. 10
    1. 100uL of 1000±10ug/mL Au standard into a 10mL volumetric flask with water
  3. 20
    1. 200uL of 1000±10ug/mL Au standard into a 10mL volumetric flask with water
  4. 30
    1. 300uL of 1000±10ug/mL Au standard into a 10mL volumetric flask with water
  5. 40
    1. 400uL of 1000±10ug/mL Au standard into a 10mL volumetric flask with water
  6. 50
    1. 500uL of 1000±10ug/mL Au standard into a 10mL volumetric flask with water

Here is the data for these samples

20140310 HRPAuNP AAdata.png

UVVis - centrifuged

I centrifuged 1mL of each sample in the centrifuge in 207 for 30 minutes at 13200rpm.

20140310 HRPAuNPc1 UVVis.png

20140310 HRPAuNPc2 UVVis.png

Notes

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