User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2013/12/05

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Objective

Setting up Protein-AuNP samples for TEM at NIST

Description

Stock solutions

  1. HAuCl4
    1. 24.6 mg
    2. MW = 393.85 g/mol
    3. Volume = 25 mL
    4. 2.5mM
  2. BSA
    1. 10.0 mg
    2. MW = 66776 g/mol
    3. Volume = 10 mL
    4. 15.0 uM
  3. Horseradish peroxidase
    1. 8.3 mg
    2. MW = 44000 g/mol
    3. Volume = 10 mL
    4. 18.9 uM
  4. Pepsin
    1. 6.9 mg
    2. MW = 34620 g/mol
    3. Volume = 10 mL
    4. 19.9 uM
  5. Myoglobin
    1. 2.7 mg
    2. MW = 16951 g/mol
    3. Volume = 25 mL
    4. 15.7 uM
  6. Lysozyme
    1. 2.5 mg
    2. MW = 17.1 g/mol
    3. Volume = 25 mL
    4. 17.1 uM
  7. hemoglobin
    1. 15.2 mg
    2. MW = 64500 g/mol
    3. Volume = 10 mL
    4. 23.6 uM
  8. Invertase
    1. 46.7 mg
    2. MW = 270000 g/mol
    3. Volume = 10 mL
    4. 16.3 uM
  9. Catalase
    1. 40.8 mg
    2. MW = 60000 g/mol
    3. Volume = 10 mL
    4. 68 uM

Samples

GoldProteinVolumes20131205 2.png

Data

These pictures were taken the following day.

BSA AuNPs20131206.jpg

Myoglobin AuNPs20131206.jpg

Myoglobin 2AuNPs20131206.jpg

LysozymeAuNPs20131206.jpg

PepsinAuNPs20131206.jpg

HbAuNPs20131206.jpg

Hb 2AuNPs20131206.jpg

InvertaseAuNPs20131206.jpg

CatalaseAuNPs20131206.jpg

HRPAuNPs20131206.jpg

Notes

This area is for any observations or conclusions that you would like to note.


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