User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2014/02/28

Biomaterials Design Lab

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Objective Learn how to maintain an OpenWetWare Notebook. Description Here is the typical synthetic protocol taken from : here. Stock solutions used were also prepared on 1/31. Synthesis Protocol for Mn-doped ZnS nanocrystals with an Mn:Zn ratio of 1:7.5 and a final (Zn+Mn) concentration of 0.4mM Add 176μL of 5mM Zn(CH3COO)2 to a test tube Add 24μL of 5mM Mn((CH3COO)2) to the test tube Add 200μL of 40mM NH4CH3COO to the test tube Add 244μL of 0.55mg/mL protein to the test tube Add 250μL of 10mM NH4OH This should bring the pH up to 8.2. Check on this with a pH strip Bring the volume up to 2.5mL with water (Add 1606μL of water) Incubate at room temperature for 1 hour Add 200μL of 5mM Na2S When you add this add it drop wise (as best as you can) while vigorously shaking the test tube. Incubate at 37C for 5 days. I made 7 different samples. 6 of these are more concentrated than the above directions. And the final sample is in a larger volume. The volumes added, shown in the table below are listed in uL, unless otherwise noted. Data Add data and results here... Notes This area is for any observations or conclusions that you would like to note. Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized. Sample Number Vol. Zn2+ Vol. Mn2+ Vol. NH4(CH3COO)2 Vol. BSA Vol. NH4OH Vol. Na2S 37.5% 500 300 800 976 1000 800 31.25% 550 250 800 976 1000 800 25% 600 200 800 976 1000 800 18.75% 650 150 800 976 1000 800 12% 704 96 800 976 1000 800 6.25% 750 50 800 976 1000 800 12% large 14.4ml 1.97ml 16.4ml 20ml 20.5ml 16.4ml and 13mL water