June 12, 2013
- Finished MHA silica-coated AuNPs, resuspended in water, 10x and 100x dilutions made for all four samples
- Fluorescent beads and Alexa 532 antibodies successfully run and analyzed on FCS
- Green fluorescent beads, diluted 10000x
- Alexa 532 antibodies, diluted to 500pM and 100pM
Notes
- Take absorbance spectra of sonicated original AuNP dilutions
- Take absorbance spectra of 10x and 100x silica-coated AuNP dilutions
- Take fluorescence spectra of ZnPPIX/AuNP, DNA/ThT combinations
- Add ZnPPIX/AuNP fluorescence data to graph for presentation
- Make DNA/ThT calibration curve (with fluorescence data)
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