User:Madeleine Y. Bee/Notebook/Single Molecule Fluorescence/2013/06/13

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June 13, 2013

Absorbance Data

Abs data AuNP dilutions 0613.png<br.> The diluted original AuNP solutions did not show absorbance spectra as expected, with the exception of the original solution(29.67nM). The samples will be remade at the same concentrations but diluted with PBS in the hopes of preventing the nanoparticles from aggregating. <br.> <br.> Abs data AuNP@SiO2 10x diluted.png>br.> The silica-coated AuNPs did not show absorbance as expected. There should be a peak between 500 and 550nm, ~520nm. <br.> <br.> Abs data AuNP@SiO2 100x diluted.png<br.> These solutions appear to be too dilute. <br.>

Notes

  • Fluormeter computer still down; fluorescence measurements to be taken as soon as possible