October 3, 2014
Procedures
New Film Synthesis
NaMT Cation Exchange
Assuming 92 miliequivalents (meq) of Na+ per 100g of montmorillonite.<br.>
- Tributylhexadecylphhosphonium bromide exchange
- Add 1.12g of Sodium Montmorillonite (NaMT) to a scintillation vial containing 20mL of 50:50 HPLC grade water:ethanol.
- (1.12 g NaMT)×(92×10-3 eq/100 g NaMT)×(507.65 g/mol tributylhexadecylphhosphonium bromide)=0.523 g tributylhexadecylphosphonium bromide
- Add 0.523g of tributylhexadecylphosphonium bromide to exchange 100% of the Na from the clay
- Cap the vial
- Stir for 1 week
- Vacuum filter using 0.2uM nylon filter paper
- Dry in oven at 90C overnight
- Grind with a mortar and pestle
- Store in a dessicator
- Choline chloride exchange
- Add 1.12g of Sodium Montmorillonite (NaMT) to a scintillation vial containing 20mL of 50:50 HPLC grade water:ethanol.
- (1.12 g NaMT)×(92×10-3 eq/100 g NaMT)×(139.62 g/mol choline chloride)=0.144 g choline chloride
- Add 0.144 g of choline chloride to exchange 100% of the Na from the clay
- Cap the vial
- Stir for 1 week
- Vacuum filter using 0.2uM nylon filter paper
- Dry in oven at 90C overnight
- Grind with a mortar and pestle
- Store in a dessicator
Dilutions and Fluoresence Measurements of R6G Solutions
- PVA film
- Original concentration: 20uM > 0.1uM (50uL in 10mL)
- Original: 10uM > 0.1uM (50uL in 10mL)
- Original: 1uM > 0.5uM (5mL in 10mL)
- PVA Clay film
- Original concentration: 20uM > 0.1uM (50uL in 10mL)
- Original: 10uM > 0.25uM (250uL in 10mL)
- Original: 1uM > 0.5uM (5mL in 10mL)
Data
Diluted solutions absorbance:<br.>
<br.>
Calculating solutions actual concentration from R6G calibration curve constructed on 10/01/2014:<br.>
|