October 1, 2014
Procedures
R6G Fluorescence: Calibration and Measurement
- Make stock concentrations (both groups can use the same solutions)
- Initial stock: 2500uM, secondary stock: 500uM
- 0.10uM
- 0.50uM
- 1.0uM
- 1.5uM
- 2.0uM
- Made with 2500uM solution
- 0.75uM
- 1.2uM
- Take UV-Vis and Fluorescence spectra of these samples
- Fluorimeter Settings:
- 500nm excitation
- 515-700nm scan range
- 10.0nm slit width
- 200nm/min scan speed
- Make a calibration curve based on UV-Vis.
- Compare your data to some published values
- Make a calibration curve based on the fluorescence.
- In order to do this, you'll need to measure the area under the fluorescence curve, not just the fluorescence peak height.
Data
Calibration Curve: R6G Absorbance
Measurement Results: R6G Absorbance
Calibration Curve: R6G Fluorescence
Measurement Results: R6G Fluoresence
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