User:Madeleine Y. Bee/Notebook/CHEM-572 2014S/2014/02/12

Myoglobin and BSA Nanofibers

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February 12, 2013 Objectives Pictures of nanofibers from yesterday: macro and micro Run tests on new nanofiber-coated scaffolds (including cell culture, stronger acid, and extended sonication) Brute force method with 0.5mM gold-Myoglobin nanofibers Autoclave one dry, one wet scaffold Myoglobin and BSA Nanofiber Results 160:1 BSA 120:1 BSA 80:1 BSA 100:1 Myoglobin 60:1 Myoglobin Nanofiber Retention Tests For 160:1, 120:1, and 80:1 BSA and 60:1 Myoglobin nanofibers Sonicate for 30 minutes Heat at 30C for 30 minutes Ethanol PBS strong acid Nanofiber Retention Test Results (February 19, 2014) (tests pictured, from left to right: strong acid, ethanol, PBS, sonicated)<br.> 160:1 BSA <br.> <br.> 120:1 BSA <br.> <br.> 80:1 BSA <br.> <br.> 60:1 Myoglobin <br.> <br.> Autoclaved Scaffolds PLA scaffolds were dry autoclaved Scaffolds did not melt as expected