February 12, 2013
Objectives
- Pictures of nanofibers from yesterday: macro and micro
- Run tests on new nanofiber-coated scaffolds (including cell culture, stronger acid, and extended sonication)
- Brute force method with 0.5mM gold-Myoglobin nanofibers
- Autoclave one dry, one wet scaffold
Myoglobin and BSA Nanofiber Results
Nanofiber Retention Tests
- For 160:1, 120:1, and 80:1 BSA and 60:1 Myoglobin nanofibers
- Sonicate for 30 minutes
- Heat at 30C for 30 minutes
- Ethanol
- PBS
- strong acid
Nanofiber Retention Test Results (February 19, 2014)
(tests pictured, from left to right: strong acid, ethanol, PBS, sonicated)<br.>
<br.>
<br.>
<br.>
<br.>
<br.>
<br.>
<br.>
<br.>
Autoclaved Scaffolds
- PLA scaffolds were dry autoclaved
- Scaffolds did not melt as expected
|