User:Madeleine Y. Bee/Notebook/CHEM-572 2014S/2014/02/12

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February 12, 2013

Objectives

  • Pictures of nanofibers from yesterday: macro and micro
  • Run tests on new nanofiber-coated scaffolds (including cell culture, stronger acid, and extended sonication)
  • Brute force method with 0.5mM gold-Myoglobin nanofibers
  • Autoclave one dry, one wet scaffold

Myoglobin and BSA Nanofiber Results

  • 160:1 BSA

Photo Feb 12, 12 17 16 PM.jpg

  • 120:1 BSA

Photo Feb 12, 12 17 31 PM.jpg

  • 80:1 BSA

Photo Feb 12, 12 17 41 PM.jpg

  • 100:1 Myoglobin

Photo Feb 12, 12 17 50 PM.jpg

  • 60:1 Myoglobin

Photo Feb 12, 12 18 06 PM.jpg

Nanofiber Retention Tests

  • For 160:1, 120:1, and 80:1 BSA and 60:1 Myoglobin nanofibers
  1. Sonicate for 30 minutes
  2. Heat at 30C for 30 minutes
  3. Ethanol
  4. PBS
  5. strong acid

Nanofiber Retention Test Results (February 19, 2014)

(tests pictured, from left to right: strong acid, ethanol, PBS, sonicated)<br.>

  • 160:1 BSA

Photo Feb 19, 2 59 58 PM.jpg<br.> <br.> Photo Feb 19, 3 00 06 PM.jpg

  • 120:1 BSA

Photo Feb 19, 2 57 38 PM.jpg<br.> <br.> Photo Feb 19, 2 57 50 PM.jpg

  • 80:1 BSA

Photo Feb 19, 2 58 48 PM.jpg<br.> <br.> Photo Feb 19, 2 58 54 PM.jpg

  • 60:1 Myoglobin

Photo Feb 19, 2 56 39 PM.jpg<br.> <br.> Photo Feb 19, 2 56 50 PM.jpg

Autoclaved Scaffolds

  • PLA scaffolds were dry autoclaved
  • Scaffolds did not melt as expected

Photo Feb 12, 2 49 20 PM.jpg