February 18, 2014
Objectives
- Polydopamine procedure for nanofibers
- Myoglobin procedure for nanofibers (repeat)
Procedures
- Preparing scaffolds
- Immerse 4 PLA scaffolds (in 5mL test tube) in ~5mL of 10mM, pH 8.5 TRIS polydopamine solution for 1 hour
- First two scaffolds immersed in solution pH 8.51
- Second two scaffolds immersed in solution pH 8.59
- Add 1mL 0.0621mM myoglobin to solution, shake test tube to combine solutions, allow surface functionalization to occur for 5 hours
- Rinse with deionized water and allow scaffold to dry overnight
Observations
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- Scaffolds after 1 hr in Tris-polydopamine
- Scaffolds after 5 hrs in Tris-polydopamine and Myoglobin solution
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