User:Madeleine Y. Bee/Notebook/CHEM-572 2014S/2014/02/05

Concentrated Nanofiber Results

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February 5, 2014 Objectives If the concentrated original nanofiber method worked with the scaffolds, Take pictures against white background Print 7 of the scaffold setup that worked and make more nanofibers according to yesterday's procedure Start tests "Brute Force" method For each nanofiber solution, there is one third of the tall scaffold Place one tube worth of nanofibers on one of the thirds (repeat for other eight scaffold thirds) Allow nanofibers to dry on the scaffolds overnight Determine method of quantification for nanofibers on the scaffolds Nanofiber Results 100:1 0.5mM Au:Myoglobin nanofibers alone<br.> <br.> 100:1 0.5mM Au:Myoglobin nanofibers on 1 tall scaffold<br.> <br.> 100:1 0.5mM Au:Myoglobin nanofibers on 4 tall scaffolds<br.> <br.> 100:1 0.5mM Au:Myoglobin nanofibers on 1 extra tall scaffold<br.> <br.> Nanofiber Retention Tests Put coated scaffold in PBS buffer Put coated scaffold in ethanol Put coated scaffold in water at 35C for 30min Put coated scaffold in water and sonicate for 30min Put coated scaffold in 0.1M HCl solution Put coated scaffold in water Pictures will be taken of sonicated and 35C after sonication and heating are complete Pictures will be taken of all solutions approximately every 2 days (Friday, Monday, and Wednesday) Nanofiber Retention Test Results: Sonicated and Heated Both samples appear unchanged 100:1 0.5mM Au:Myoglobin nanofiber coated scaffold, sonicated 30minutes<br.> <br.> 100:1 0.5mM Au:Myoglobin nanofiber coated scaffold, heated at 35C for 30minutes<br.> <br.>