User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/09/17

Experimental Biological Chemistry: Fall 2013

Main project page Previous entry      Next entry

September 17, 2013 Objective Execute the following procedure from Dr. Hartings' notebook, in order to determine the amount of potassium ferricyanide/sodium dithionite that is required to completely oxidize/reduce (respectively) horseradish peroxidase. Data Oxidation with Potassium ferricyanide<br.> <br.> <br.> Reduction with Sodium dithionate<br.> <br.> Notes Results for both the oxidation and reduction were not observed as expected. This may be because the horseradish peroxidase oxidized/reduced by the first measurement, and subsequent measurements showed no change in absorbance because of this.<br.> Once in each trial, the solution was spiked with more concentrated reagent, which showed very little change as well, validating the above conclusion. [[Category:]]