User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/09/17

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September 17, 2013

Objective

Execute the following procedure from Dr. Hartings' notebook, in order to determine the amount of potassium ferricyanide/sodium dithionite that is required to completely oxidize/reduce (respectively) horseradish peroxidase.

Data

Oxidation with Potassium ferricyanide<br.> 2013 0917 HRP oxidation spectra.PNG<br.> <br.>

Reduction with Sodium dithionate<br.> 2013 0917 HRP reduction spectra.PNG<br.>

Notes

Results for both the oxidation and reduction were not observed as expected. This may be because the horseradish peroxidase oxidized/reduced by the first measurement, and subsequent measurements showed no change in absorbance because of this.<br.> Once in each trial, the solution was spiked with more concentrated reagent, which showed very little change as well, validating the above conclusion.


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