User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/09/18
|Experimental Biological Chemistry: Fall 2013||Main project page|
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September 18, 2013
Follow this procedure to perform a redox titration for Horseradish peroxidase and determine it's standard reduction potential.
The redox reaction will be monitored with a spectroelectrochemical cell, and UV-vis measurements will be taken to observe the resulting redox state. DTT will be added in 1uL increments and the UVvis and circuit potential data will be recorded, to eventually plot %oxidized or %reduced versus voltage. <br.>