User:Madeleine Y. Bee/Notebook/CHEM-571 2013F/2013/09/11

Experimental Biological Chemistry: Fall 2013

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September 11, 2013 Objective Take Atomic Absorption measurements for gold standards, BSA-AuNPs, and citrate-AuNPs. Construct a calibration curve with the gold standards data. Procedures Atomic absorption measurements taken of the following samples Gold Standards: 5ug/mL 10ug/mL 15ug/mL 20ug/mL 25ug/mL BSA-AuNPs Citrate-AuNPs 10x dilution Water Data Calibration Curves Group Data<br.> <br.> <br.> Class Data<br.> <br.> Calculations Determine the number of Au atoms per nanoparticle [Au]from AA class calibration data=74.45ug/mL Converted to mmol/L= 0.37798mM [AuNP] from UV-vis determination= 8.349nM [Au]/[AuNP]=(3.7798×10-4M)/(8.349×10-9M)=45272.49 Au/AuNP<br.>