User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/05/26

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Today's Benchwork

  1. Miniprep of O/N culture
    • Follow quick vacuum protocol, except:
      • At step 5, added 750 μL instead of 350 μL Neutralization solution. I "corrected" this by adding an additional 200 μL Cell Lysis solution before step 6, thus increasing the total volume.
  2. Quantify [ pCMV-SPORT6+BSA ]
    1. 95μL H2O
    2. + 5μL miniprepped DNA from step 1
    • → measure absorbance at 260 nm and 280 nm
  3. PCR of BSA for cloning into pTXBI

Results

Failed to parse (MathML with SVG or PNG fallback (recommended for modern browsers and accessibility tools): Invalid response ("Math extension cannot connect to Restbase.") from server "https://api.formulasearchengine.com/v1/":): {\displaystyle [CMV-SPORT6+BSA]=(0.130AU+0.028AU)\times50\frac{\frac{\mu g}{mL}}{AU}\times\frac{100\mu L}{5\mu L}=158\frac{\mu g}{mL}}

Failed to parse (MathML with SVG or PNG fallback (recommended for modern browsers and accessibility tools): Invalid response ("Math extension cannot connect to Restbase.") from server "https://api.formulasearchengine.com/v1/":): {\displaystyle \frac{A_{260}}{A_{280}}=\frac{0.130AU+0.028AU}{0.055AU+0.029AU}=1.88}