User:Kathryn Muratore/Notebook/AU CHEM-570 lab prep/2011/05/27

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Objective

  • Confirm that PCR worked and product is consistent with expectations. Also confirm that miniprepped plasmid is consistent with expectations.

Bench work

  1. Analytical digest of pCMV-SPORT6+BSA] with XmnI
  2. Analytical minigel
    • 1.2% Agarose gel (~20 mL with small-tooth comb)
    • Mix samples with 6X DNA loading buffer and load:
    1. 10μL 1KB ladder
    2. 10μL XmnI-digested plasmid from step 1
    3. 5μL PCR product from yesterday, step 3

Results and Conclusions

DNA gel 05.27.11.jpg Results are consistent with expectations.