User:Karmella Haynes/Notebook/BioBrick cloning/2012/12/13

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12/13/12

  • Mnipreps & Digests: KAH111/C-His clones



Minipreps

  • Check with E/P digests
Reagent Volume Mix (x9) Expected:
1-8. KAH111/C-His = 3600, 1123
Empty C-His = 3600
Blank gel! (only ladder)
15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 2.0 ---
10X buffer 1.5 13.5
EcoRI 1.0 9.0
PstI 1.0 9.0
dH2O 9.5 85.5
  15 μL

--> 37°C/ ~15 min.