User:Karmella Haynes/Notebook/BioBrick cloning/2012/12/01

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.pngKarmella's BioBrick Cloning Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

12/01/12

  • Cultures/ minipreps: KAH3/MV2 colonies (2)



Minipreps

  • Check with PstI digest
  • Compare to empty vector MV2
Reagent Volume Expected:
1. MV2 = 4529
2. KAH3/MV2-1 = 5280
3. KAH3/MV2-2 = 5280
Hover name
15 μL/lane; 1% agarose; First pair of digests have 3.0 μL plasmid. Ladder
DNA(plasmid) 2.0 μL
10X buffer 1.5
EcoRI 1.0
PstI 1.0
dH2O 9.5
  15.0 μL
37°C/ ~15 min.