User:Karmella Haynes/Notebook/BioBrick cloning/2012/12/03

Karmella's BioBrick Cloning

Main project page Previous entry      Next entry

12/03/12 Assembly: KAH111 + C-His expression vector (trouble shooting for Behzad) Assemblies KAH111/C-His: KAH111/(E/P)/1123 + C-His/(E/P)/3600 Digests (Fermentas FD) Already done by Behzad Measure conc.'s Behzad: KAH111 = 15 ng/μL Behzad: C-His = 57 ng/μL Ligations Ligation Plate results (lig : neg crtl) 12/04/12 1. KAH111(E/P)/1123, 16 ng + C-His(E/P)/3600, 25 ng KAH111/C-His 0 colonies 2. C-His(E/P)/ 25 ng 0 colonies   1 2 Insert DNA 1.0 --- Vector DNA 0.5 0.5 2x lgn buf (Roche) 5.0 5.0 T4 ligase (NEB) 1.0 1.0 dH2O 2.5 3.5   10 μL 10 μL