User:Jessie Wang Lectures Mod1
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Lecture 0: Intro to 20.109 (9/6)
Lecture 1: Intro to Genome Engineering (9/11)
Lecture 2: Genes as Parts (9/13)
Lecture 3: Synthetic Biology (9/18)
Genetic Engineering vs. Synthetic Biology
Genetic Engineering Tools:
1. rDNA (1970s) - cut and paste pre-existing DNA fragments
2. PCR (1985) - amplify and modify exiting DNA
3. Sequencing (1977) - read DNA into A,T,C,G
Synthetic Biology:
4. Automated Construction (2004) - DNA synthesis (1987)
5. Standards (2001)
6. Abstraction (2003)
Functional Composition
Switch - bistable
GDP - | G-Protein | <--> GTP - | G-Protein |
Riboswitches
- Ribozyme
- Aptamer
- Computation Domain
Physical Composition
M13
Lecture 4
Construction
- Phosphoramidites - (A,T,C,G)
- Came from salmon milt.
- 10 g x 1 mol/1000 g x 6E23 molecules/mol = 6 x 10^21 molecules of a particular base
- 10^10 people/Earth x 10^10 bp/person = 10^20 bp/Earth
- DNA synthesis
- Deprotect
- Coupling
- Oxidation
- Capping