User:Jeffrey Kim/Notebook/Halogenated natural products from uncultured bacteria/2009/01/11

Halometabolites from uncultured bacteria	Main project page Previous entry      Next entry
TAR cloning setup Performed recombinational cloning on uracil dropout plates (blobel lab) with two 50 ml aliquots of chemically competent yeast prepared that morning from a 1:100 dilution of an O.N culture at 30C. 5 hours of incubation yielded cells of OD 0.4 for preparation. 200ul of competent yeast were prepared per 50ml culture and plated onto the uracil dropout plates.