User:Jeffrey Kim/Notebook/Halogenated natural products from uncultured bacteria/2009/01/12

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BB16 metabolite extraction

  • Streptomyces Albus cultures (pOJ436 negative control, BB16-1, BB16-2) were grown with shaking at 30C in 150ml SMM. 25ml were ethyl acetate extracted in Falcon tubes for lyophilization, and TLC analysis. 25ml will be methanol water (95/5) extracted, lyophilized, and analyzed as well after methanol resuspension. Aqueous phases after partitioning were combined and dried down in the speed vac for HILIC analysis.
  • pH alterations will potentially be tested in parallel to see if it helps in identification.