User:Hannah Harvey/Notebook/CHEM-471 Fall 2016/2016/10/04

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Objectives

  1. Run fluorescence data for lysozyme at pH=7
  2. Create stock solutions for use on 10.05.2016

Description

Fluorescence

  1. Solutions made using measurements below
  2. Fluorescence data run every 3 minutes for 3 hours

Solutions for 10/05/2016
Solutions were added to glass test tubes, covered with foil, and placed in an oven set to 80°C for 4 hours.

Data

100416 Emit vs Time pH 7.png

Figure 1 Maximum emission data in a solution of pH 7.

100416 Int v Time Lys pH 7.png

Figure 2 Integrated fluorescence data in a solution of pH 7.

100416 Full Fluor Lys pH 7.png

Figure 3 Full emission spectra for important time points: t=0, 15, 33, 105, 180 min.

Figure 3 Explanation

  1. At t=0 min, we see a max emission far to the left of the other emission spectra.
  2. At t=15 min, this maximum has shifted up and to the right.
  3. Until t=33 min, the maximum for each spectra decreases until the shown maximum.
  4. After t=33 min, the maximum begins increasing again.
  5. At t=105 min, the maximum continues to increase, but the "shoulder" seen at around 475 nm begins going away.
  6. t=180 shows the final full spectra obtained.

UV-vis solutions for 09.14.2016
AgNP Fruct 0mM Lys UVvis.png
Table 1 UV-vis solutions made for [Fructose]=.0mM with silver

Image-AgNP Fruct .0625mM Lys UVvis.png.png
Table 2 UV-vis solutions made for [Fructose]=.0625mM with silver