User:Hannah Harvey/Notebook/CHEM-471 Fall 2016/2016/10/05

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Objectives

  1. Run UV-vis for 18 samples with lysozyme as the protein. Full concentration details can be found below under Data.

Description

  1. Test solutions made according to below concentrations under Data.
  2. On 10/04, solutions were placed into the oven at 80°C for 4 hours.
  3. UV-vis data was run on each of the 18 samples of different pHs and fructose concentrations.
  4. After each individual sample was run, the sample was saved and the cuvette cleaned out twice with soap, twice with H2O, twice with HCl, and twice more with H2O. The cuvette was then dried thoroughly.

Data

Solution concentrations
100516 Lys UVvis Conc Fruc 0mM.png
Table 1 UV-vis solutions made for [Fructose]=0 mM

100516 Lys UVvis Conc Fruc .0625mM.png
Table 2 UV-vis solutions made for [Fructose]=.0625 mM


100516 Abs v Wave Fruct 0.png

Figure 1 Absorbance v wavelength of lysozyme in 0 mM concentration of fructose

100516 Abs v pH Fruct 0mM.png

Figure 2 Absorbance at 281 nm at various pH's in 0 mM concentration of fructose

100516 Abs v Wave Fruct .0625mM.png

Figure 3 Absorbance v wavelength of lysozyme in 0.0625 mM concentration of fructose

100516 Abs v pH Fruct .0625 mM.png

Figure 4 Absorbance at 281 nm at various pH's in .0625 mM concentration of fructose

Notes

All Figures in the above data are baseline and water corrected.
After taking the UV-vis samples out of the 80°C oven, pH 11 for both Fructose concentrations showed a precipitate form. pH 12 did not show this precipitate. The pictures of each precipitate are shown below:

10.05.16 UVvis Fruc 0mM.jpg
Figure 5 Solid formed in [Fructose]=0 mM

10.05.16 UVvis Fruc .0625mM.jpg
Figure 6 Solid formed in [Fructose]=.0625 mM