User:Hannah Harvey/Notebook/CHEM-471 Fall 2016/2016/09/20

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Objective

  1. Run Ocean Optics for Lysozyme protein with a pH of 5.

Description

  1. Make solution for cuvette. Concentrations can be found in the Data section below.
  2. Run Ocean Optics data every two minutes for 3 hours.

Data

Stock solutions

  1. Lysozyme
    1. concentration = 225 μM
  2. AuCl3
    1. concentration = 3.73 mM

Instructions for making sample for ocean optics

  1. pH 5
    1. Lysozyme: 0.25mM Au, 12.5μM Lysozyme, 3mL total
      1. 223 μL AuCl3
      2. 30 μL 1M HCl
      3. 167 μL Lysozyme
      4. 2580 μL Water
092016 Wave v time OO Lys pH5.png

Figure 1 Values taken with lysozyme at a pH of 5

092016 Abs v time Lys OO pH5.png

Figure 2 Values taken with lysozyme at a pH of 5

092016 Abs v Wave Lys OO pH5.png

Figure 3 Absorbance v wavelength Ocean Optics data for lysozyme at a pH of 5. Values shown every 30 minutes. In addition, values shown at t=46-48 due to the large jump observed in Figure 1