User:Hannah Harvey/Notebook/CHEM-471 Fall 2016/2016/09/20

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Objective Run Ocean Optics for Lysozyme protein with a pH of 5. Description Make solution for cuvette. Concentrations can be found in the Data section below. Run Ocean Optics data every two minutes for 3 hours. Data Stock solutions Lysozyme concentration = 225 μM AuCl3 concentration = 3.73 mM Instructions for making sample for ocean optics pH 5 Lysozyme: 0.25mM Au, 12.5μM Lysozyme, 3mL total 223 μL AuCl3 30 μL 1M HCl 167 μL Lysozyme 2580 μL Water Figure 1 Values taken with lysozyme at a pH of 5 Figure 2 Values taken with lysozyme at a pH of 5 Error creating thumbnail: Unable to save thumbnail to destination Figure 3 Absorbance v wavelength Ocean Optics data for lysozyme at a pH of 5. Values shown every 30 minutes. In addition, values shown at t=46-48 due to the large jump observed in Figure 1