Figure 1 Maximum emission data of tryptophan in a solution of pH 5.
Figure 2 Integrated fluorescence data of tryptophan in a solution of pH 5.
Figure 3 Full emission spectra for important time points: t=0, 5, 35, 90, 180 min.
Figure 3 Explanation
At t=0 min, we see a max emission far to the left of the other emission spectra.
At t=5 min, this maximum has shifted up and to the right.
Until t=35 min, the maximum for each spectra decreases until the shown maximum.
After t=35 min, the maximum begins increasing again.
At t=90 min, the maximum continues to increase, but the "shoulder" seen at around 475 nm begins going away.
t=180 shows the final full spectra obtained.
UV-vis solutions for 09.14.2016 Table 1 UV-vis solutions made for [Fructose]=.125mM
Table 2 UV-vis solutions made for [Fructose]=.25mM
Notes
At t=0, the cuvette had been in the machine briefly for about 1-2 minutes before the machine was started, which could account for some error in the data.
While running the fluorescence data, the machine briefly shut off at the 100 minute mark. The fluorescence data shows t=95, t=103, t=105 instead.
After taking the UV-vis samples out of the 80°C oven, pH 11 for both Fructose concentrations showed a precipitate form. pH 12 did not show this precipitate.