User:Hannah Harvey/Notebook/CHEM-471 Fall 2016/2016/09/07

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Objective

  1. Run Ocean Optics for Lysozyme protein with a pH of 4.

Description

  1. Make solution for cuvette. Concentrations can be found in the Data section below.
  2. Run Ocean Optics data every two minutes for 3 hours.

Data

Stock solutions

  1. Lysozyme
    1. concentration = 62.1 μM
  2. AuCl3
    1. concentration = 5.08 mM

Instructions for making sample for ocean optics

  1. pH 4
    1. Lysozyme: 0.25mM Au, 12.5μM Lysozyme, 3mL total
      1. 147 μL AuCl3
      2. 300 μL 1M HCl
      3. 245 μL Lysozyme
      4. 2.308 mL Water
090716 Wave v Time LyspH4.png

Figure 1 Values taken with lysozyme at a pH of 4

090716 Abs v Time OO Lys pH4.png

Figure 2 Values taken with lysozyme at a pH of 4

090716 Abs v Wave Lys.png

  1. Figure 3 Absorbance v wavelength Ocean Optics data for lysozyme at a pH of 4. Values taken every 30 minutes.