Objective
- Run Ocean Optics for Lysozyme protein with a pH of 4.
Description
- Make solution for cuvette. Concentrations can be found in the Data section below.
- Run Ocean Optics data every two minutes for 3 hours.
Data
Stock solutions
- Lysozyme
- concentration = 62.1 μM
- AuCl3
- concentration = 5.08 mM
Instructions for making sample for ocean optics
- pH 4
- Lysozyme: 0.25mM Au, 12.5μM Lysozyme, 3mL total
- 147 μL AuCl3
- 300 μL 1M HCl
- 245 μL Lysozyme
- 2.308 mL Water
Figure 1 Values taken with lysozyme at a pH of 4
Figure 2 Values taken with lysozyme at a pH of 4
![](https://s3-us-west-2.amazonaws.com/oww-files-public/8/8f/090716_Abs_v_Wave_Lys.png)
- Figure 3 Absorbance v wavelength Ocean Optics data for lysozyme at a pH of 4. Values taken every 30 minutes.
|