- Took beads out of freeze dryer
- Boiled cabbage leaves
- Swelled Beads
- Freeze dried beads were black and clumped into solid rocks. Potentially the lower pressure was enough to let the hydrolysis reaction happen.
- Places beads in containers with enough water to coat them
- 10% Beads: 0.811 g
- 25% Beads: 1.007g
- 40% Beads: 1.123g
- Were in solid clumps
- Poking them with a stick and shaking them did not separate them into beads
- Beads were a blackish-greenish color
- Upon placing the beads into water they separated a little into beads and turned a lighter green color
- After about half an hour of sitting in water, water solution had a pH of about 1 according to pH test.
- 40% beads were fasted to turn green.
- Turned a richer, greener green
- 25% Beads took longest to turn green
- Color somewhat between 40% and 10%
- Turned a yellower green color (more like seaweed)
- Boiled dye until most of pigmentation came out
- Checked effect of pH on color
- Dye is neutral
- Checked effect of pH on dye color
- Beaker on the left is acidic. Beaker in the middle is neutral. Beaker on the right is basic.
- Ran UV-Vis on Dye and Serial Dilutions as follows: Stock solution, 4:1 Stock to water, 3:2 Stock to water, 2:3 Stock to water, and 1:4 Stock to water.
- Neutral Dye absorbed between 545-548 nm
- Got the following UV-Vis Spectrum:
- Made trendline for absorbance of serial dilutions. Percentages are based of stock solution. 100% = same concentration as stock solution. 20% is 1:4 stock to dye. Etc.
Link to patent that explains discoloration in PVA