User:Desiree Carmen/Notebook/AU Biomaterial Lab/2014/02/28

Biomaterials Design Lab

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Objective Synthesize Mn-doped ZnS quantum dots using the synthesis described here Description Synthesis Protocol for Mn-doped ZnS nanocrystals with an Mn:Zn ratio of 1:7.5 and a final (Zn+Mn) concentration of 0.4mM- Final solution is 5mL. All amounts of solution are doubled on this protocol. Add 176μL of 5mM Zn(CH3COO)2 to a test tube Add 24μL of 5mM Mn((CH3COO)2) to the test tube Add 200μL of 40mM NH4CH3COO to the test tube Add 244μL of 0.55mg/mL protein to the test tube Add 250μL of 10mM NH4OH This should bring the pH up to 8.2. Check on this with a pH strip Bring the volume up to 2.5mL with water (Add 1606μL of water) Incubate at room temperature for 1 hour Add 200μL of 5mM Na2S When you add this add it drop wise (as best as you can) while vigorously shaking the test tube. Incubate at 37C for 5 days. Variations for 4 Test Tubes DC1 Add 176μL of 5mM Zn(CH3COO)2 to a test tube Add 24μL of 5mM Mn((CH3COO)2) to the test tube DC2 Add 150μL of 5mM Zn(CH3COO)2 to a test tube Add 50μL of 5mM Mn((CH3COO)2) to the test tube DC3 Add 160μL of 5mM Zn(CH3COO)2 to a test tube Add 40μL of 5mM Mn((CH3COO)2) to the test tube Add 30μL extra of 10mM NH4OH to final solution to bring pH to 8.2. Add only 3182μL of water to bring to 5mL instead of 3212μL. DC4 Add 170μL of 5mM Zn(CH3COO)2 to a test tube Add 30μL of 5mM Mn((CH3COO)2) to the test tube Data Stock Solutions All solutions are made in 250mL volumetric flasks. 5mM Zn(CH3COO)2 FW = 219.49 g/mol V = 250mL mass to be measured = 0.274g actual mass = 0.274g actual concentration = 5mM 5mM Mn(CH3COO)2 FW = 173.02 g/mol V = 250mL mass to be measured = 0.216g actual mass = 0.220 g (slightly pinkish hue) actual concentration = 5.1mM 40mM NH4(CH3COO)2 FW = 77.08 g/mol V = 250mL mass to be measured = 0.7708g actual mass = 0.780g actual concentration = 40.5mM 10mM NH4OH initial M = 14.5M final V = 250mL volume to be diluted = 172μL actual concentration = 5mM Na2S FW = 240.18 g/mol V = 250mL mass to be measured = 0.300g actual mass = 0.300g actual concentration = 5.00mM Notes All solutions were doubled to make a solution of 5mL. 5mM Na2S added at 12:45pm after hour incubation. Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.