Cluzel Lab Notebook
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Prepping Plasmid for Electroporation
PZE-12-CFP vector from JM Kim
Doing 4 electroporations, in order to ensure competent cells made last week work correctly.
Protocol steps on page 1.119 - 1.122 of Molecular Cloning, Volume 1 book
Put in 37 degree shaker w/ the SOC for 1 hour. Spun down, supernatant removed to ~50ul each, resuspended, plated and spun onto correct antibiotic plates w/ LB. Put in 37 degree incubator overnight at 7:45 pm. Will take out tomorrow morning.