Printing spots on glass slide using micrometer stage
- printed from 10.5-11.1 Y axis
- printing produces reproducible spots, smaller than chip width (need to compute exact diameter)
- printing from higher up might reduce spot size, need to check
- printing repeatedly might reduce spot size
- need to quantify OD:cells per area ratio (might change with stationary vs exponential
- phase and 1 gfp image saved in 4-2 dir on titan
- will transfer to agarose later today: saved slide in petri dish at 12:15 pm
Agarose
- made 1 ml 4% agarose at 12:20 pm
Cells
- grew 1 ml cells with 1 col gfp cells (around 11:45 am); forgot to add amp (1 ul), did so at 12:22 pm
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