September 14, 2016 : UV-Vis II
Objective
- Run UV-Vis on gold nanoparticle solutions prepped 09.13.16
Description
UV-Vis
- Solutions prepped on 09.13.16 were collected for use on UV-Vis
- Each solution, individually, was added to the cuvette
- The cuvette was added to the UV-Vis machine and run from 800nm to 200nm
- Once the run was complete the cuvette was cleaned with 2 soap, 2 water, 2 HCl and 2 water flushes. Followed by a thorough drying.
Data
0.125 mM Fructose
pH
|
μL of 1 mM HClstock
|
μL of 1 mM NaOHstock
|
μL of 1 M NaOHstock
|
μL of AuCl3stock
|
μL of Fructosestock
|
μL of Lysozymestock
|
μL of Water
|
4 |
500 |
0 |
0 |
274 |
48 |
268 |
3910
|
5 |
50 |
0 |
0 |
274 |
48 |
268 |
4360
|
6 |
5 |
0 |
0 |
274 |
48 |
268 |
4405
|
7 |
0 |
0 |
0 |
274 |
48 |
268 |
4410
|
8 |
0 |
5 |
0 |
274 |
48 |
268 |
4405
|
9 |
0 |
50 |
0 |
274 |
48 |
268 |
4360
|
10 |
0 |
500 |
0 |
274 |
48 |
268 |
3910
|
11 |
0 |
0 |
5 |
274 |
48 |
268 |
4405
|
12 |
0 |
0 |
50 |
274 |
48 |
268 |
4360
|
0.25 mM Fructose
pH
|
μL of 1 mM HClstock
|
μL of 1 mM NaOHstock
|
μL of 1 M NaOHstock
|
μL of AuCl3stock
|
μL of Fructosestock
|
μL of Lysozymestock
|
μL of Water
|
4 |
500 |
0 |
0 |
274 |
96 |
268 |
3862
|
5 |
50 |
0 |
0 |
274 |
96 |
268 |
4312
|
6 |
5 |
0 |
0 |
274 |
96 |
268 |
4357
|
7 |
0 |
0 |
0 |
274 |
96 |
268 |
4362
|
8 |
0 |
5 |
0 |
274 |
96 |
268 |
4357
|
9 |
0 |
50 |
0 |
274 |
96 |
268 |
4312
|
10 |
0 |
500 |
0 |
274 |
96 |
268 |
3862
|
11 |
0 |
0 |
5 |
274 |
96 |
268 |
4357
|
12 |
0 |
0 |
50 |
274 |
96 |
268 |
4312
|
Notes
Samples at pH 11 contained precipitate.
|