User:Cosette Brianna Taggart/Notebook/Experimental/2016/09/20

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September 20, 2016 : Ocean Optics II

Objective

  1. Run Ocean optics on sample prepped today.

Description

Ocean Optics

  1. Sample was prepped according to Lysozyme prep seen in data
  2. Sample was placed into Ocean Optics Machine
  3. Machine was run for 3 hours collecting data every 2 minutes

Data

Stock solutions

  1. Lysozyme
    1. 32.24 mg of lysozyme with water in 10.0 mL volumetric flask
    2. MW = 14307 g/mol
    3. concentration = 225 μM
  2. AuCl3
    1. 28.27 mg of AuCl3 with water in 25.0 mL volumetric flask
    2. MW = 303.3 g/mol
    3. concentration = 3.73 mM
  1. pH 5
    1. Lysozyme: 0.25mM Au, 12.5μM Lysozyme, 3mL total
      1. 223 μL AuCl3
      2. 30 μL 1M HCl
      3. 167 μL Lysozyme
      4. 2580 μL Water


Summary Data at 30 minute increments
Time(minutes) Maximum Absorbance Maximum Wavelength(nm)
0 0.13438 428
30 0.14248 428
60 0.28637 538.98
90 0.45071 535.5
120 0.55944 538.98
150 0.63036 534.45
180 0.67341 535.5


MaximumAbsorbance092016.png

MaximumWavelength092016.png

092016 Abs v Wave Lys OO pH5.png

Notes