September 20, 2016 : Ocean Optics II
Objective
- Run Ocean optics on sample prepped today.
Description
Ocean Optics
- Sample was prepped according to Lysozyme prep seen in data
- Sample was placed into Ocean Optics Machine
- Machine was run for 3 hours collecting data every 2 minutes
Data
Stock solutions
- Lysozyme
- 32.24 mg of lysozyme with water in 10.0 mL volumetric flask
- MW = 14307 g/mol
- concentration = 225 μM
- AuCl3
- 28.27 mg of AuCl3 with water in 25.0 mL volumetric flask
- MW = 303.3 g/mol
- concentration = 3.73 mM
- pH 5
- Lysozyme: 0.25mM Au, 12.5μM Lysozyme, 3mL total
- 223 μL AuCl3
- 30 μL 1M HCl
- 167 μL Lysozyme
- 2580 μL Water
Summary Data at 30 minute increments
Time(minutes)
|
Maximum Absorbance
|
Maximum Wavelength(nm)
|
0 |
0.13438 |
428
|
30 |
0.14248 |
428
|
60 |
0.28637 |
538.98
|
90 |
0.45071 |
535.5
|
120 |
0.55944 |
538.98
|
150 |
0.63036 |
534.45
|
180 |
0.67341 |
535.5
|
Notes
|