User:Cosette Brianna Taggart/Notebook/Experimental/2016/09/13

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September 13, 2016 : Fluorescence II

Objective

  1. Collect fluorescence data for lysozyme at pH 5
  2. Make stock solutions for UV-Vis on 9.14.2016

Description

Fluoresence

  1. Lysozyme solution was made according to the measurements found in data below
  2. Lysozyme solution was run through florescence for 3 hours with data taken every 5 minutes

Stock Solutions for 9.14.2016

Solutions were made in glass test tubes, covered with foil, and placed in an oven for 4 hours at 80 C.

Data

Fluorescence

  1. Solution Measurements
    1. Lysosome Stock Solution
      1. 33.32 mg of lysozyme with 10.0 mL of water
      2. MW = 14307 g/mol
      3. concentration = 233 μM
    2. Gold Stock Solution
      1. 34.62 mg of AuCl3 with 25.0 mL of water
      2. concentration = 4,57 mM
    3. Lysozyme: 0.25mM Au, 12.5μM Lysozyme, 3mL total; pH 5
      1. 164 μL AuCl3
      2. 30 μL 1M HCl
      3. 161 μL Lysozyme
      4. 2645 μL Water

SUMMARY DATA AND FIGURES

Summary Data at 15 minute increments
Time(minutes) Maximum Emission Integrated Intensity
0 409 4906.227857
15 416.5 6811.555683
30 422.5 6130.057445
45 423 6092.256217
60 428 6412.511527
75 427 6737.149697
90 424.5 7059.65793
105 430 7475.010129
120 426.5 7766.640525
135 428 8467.028669
150 425.5 8702.320804
165 427.5 8902.340656
180 426 9136.602808


20160913LysozymepH5II.png

20160913LysozymepH5.png

091416 Fluorescence Summary Lysozyme pH5.png

Notes

Machine disconnected for the 100 min reading. Data was taken at 103 mins and continued in 5 minute increments @ time 105 min.