User:Cosette Brianna Taggart/Notebook/Experimental/2016/09/13

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September 13, 2016 : Fluorescence II Objective Collect fluorescence data for lysozyme at pH 5 Make stock solutions for UV-Vis on 9.14.2016 Description Fluoresence Lysozyme solution was made according to the measurements found in data below Lysozyme solution was run through florescence for 3 hours with data taken every 5 minutes Stock Solutions for 9.14.2016 Solutions were made in glass test tubes, covered with foil, and placed in an oven for 4 hours at 80 C. Data Fluorescence Solution Measurements Lysosome Stock Solution 33.32 mg of lysozyme with 10.0 mL of water MW = 14307 g/mol concentration = 233 μM Gold Stock Solution 34.62 mg of AuCl3 with 25.0 mL of water concentration = 4,57 mM Lysozyme: 0.25mM Au, 12.5μM Lysozyme, 3mL total; pH 5 164 μL AuCl3 30 μL 1M HCl 161 μL Lysozyme 2645 μL Water SUMMARY DATA AND FIGURES Summary Data at 15 minute increments Time(minutes) Maximum Emission Integrated Intensity 0 409 4906.227857 15 416.5 6811.555683 30 422.5 6130.057445 45 423 6092.256217 60 428 6412.511527 75 427 6737.149697 90 424.5 7059.65793 105 430 7475.010129 120 426.5 7766.640525 135 428 8467.028669 150 425.5 8702.320804 165 427.5 8902.340656 180 426 9136.602808 Notes Machine disconnected for the 100 min reading. Data was taken at 103 mins and continued in 5 minute increments @ time 105 min.