User:Anthony Salvagno/Notebook/Research/2009/10/29/Redoing Unzipping: Gel of Redone Anchor PCR
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Gel Results
SJK 12:37, 29 October 2009 (EDT)
The image is blurry due to fog on the filter. I really should figure out some way to eliminate that. Anyways here is the lane readout:
- 1x Ladder
- 2x Ladder
- Tube 1
- Tube 2
- Tube 3
- Tube 4
See yesterday for contents of tube
It seems like Tube 1 almost worked and Tube 4 definitely worked. Tubes 2 and 3 definitely did not work. What happened in tube 1? Maybe extension is too long. I had this result happen to me before. I definitely think this is a thermal cycler thing now. There is relatively no difference in these reactions outside of the primers, and their locations are at most 100bp apart. I think the reaction worked depending on where I put them in the thermal cycler. I def need to take measurements. Anyways