User:Anthony Salvagno/Notebook/Research/2009/03/11/Official T'form Results

Results and Comments

We made 5 plates.

^{SJK 17:12, 11 March 2009 (EDT)}

17:12, 11 March 2009 (EDT)
For plate 1, what does "some background DNA" mean? Did you mean to say some background colonies?

Gel Prep

Plate Number	Contains	Comments
1	vector only	some background DNA, this was expected
2	insert only	nothing, good result
3	both vector and insert (1:1 V:I)	nothing, bad result
4	both vector and insert (2:1 V:I)	1 colony, funny result but still bad
5	nothing but E coli cells	nothing, good display of sterile technique

Growth

We took that one colony and used it. We figured we might as well. I didn't want to waste all that effort I had put in.
Method:

1. I plucked the colony
2. Placed colony into LB media
3. Place tube into shaker overnight
4. Watch it grow.