User:Anthony Salvagno/Notebook/Research/2009/03/11

From OpenWetWare
Jump to: navigation, search
Chef2.png Research Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

/Digestion, Ligation, Transformation Retry Day 1

As I mentioned yesterday, the transformation failed probably during the ligation step of the process. Kelly (and Mary Ann) believes that there were too many small fragments compared to ligatable plasmid DNA so there may have been a lot of gDNA self ligation going on. We need to redo the process all over again...

/Official T'form Results

See here for previous comments.