User:Anneliese Faustino/Notebook/671 Nanoparticles/2016/10/18

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Sample Prep

BSA Ocean Optics Solution

  1. pH 8
  2. [Au]=0.25 mM
  3. [BSA]=3.125 uM
  4. Vf=3000 uL

Stock Concentrations 160920

  1. [BSA]= 63.1 uM
  2. [Au]= 2.39 mM
  3. [NaOH]= 1 mM

Volumes for making the solution:

  1. 148.9 uL BSA
  2. 255.6 uL Au
  3. 2565.4 uL HOH
  4. 3 uL NaOH

Results and Data Analysis

OO 18 meme part 1.PNG

The above chart displays the absorbance at 535.15 nm of the pH 8 solution with 0.25 mM Au and 3.125 uM BSA over time. The chart shows a gradual increase in absorbance over the run time.

OO 18 meme part 2.PNG

The above chart shows the maximum wavelength (nm) of the pH 8 solution with 0.25 mM Au and 3.125 uM BSA over time. The chart displays a plateau of max wavelength ~485 nm until about ~3800 seconds, where it jumps dramatically to about ~550 nm; then leveling off between 530 and 540 nm.

OO 18 meme part 3.PNG

The above chart shows the gradual increase in the maximum absorbance of the pH 8 solution with 0.25 mM Au and 3.125 uM BSA over time.